Development of an herbal syrup using leaves extract of crassocephalum crepidioides
Keywords:Herbal formulations,syrup,physico chemical analysis, c.crepidioides.
The leaves of c.crepidioides is reported to have good medicinal values and used in treatment for various ailments in traditional medicine system. The present study was undertaken with an aim of developing an herbal syrup by using c.crepidioides leaves extract and to study overall acceptibility, to perform physico chemical analysis, and to study the shelf life of the develop syrup. There was four different formulations. First formulation consist of only leaves extract and sugar syrup in equal amount, second formulation consists of sugar syrup, leaves extract and orange juice in the ratio of 50:25:25, the third formulation consists of sugar syrup ,leaves extract and pineapple juice in the ratio of 50:25:25 and the last i.e. fourth formulation consists of sugar syrup, leaves extract and both the orange and pineapple juice in 50:25:12.5:12.5 ratio. Acceptibility trials of four sample were conducted by semi trained panels using 9 point hedonic scale and based on that four sample were accepted for further analysis. Storage of all the four sample were done by keeping them in air tight container made of plastic and glass at room temperature. Assessment of moisture content of the dry leaves of c.crepidioides was carried out and the result obtained was 4% w/w. The phytochemicals screening was also performed to screened some phytochemicals and flavonoid, phenol, tannin and alkaloid were found to be present. The DPPH free radical scavenging activity was investigated for methanolic extract of c.crepidioides and the result of the laboratory analysis show the IC50 value 2.64±0.019. Further the leaves was studied for its phenolic content and the result was 0.111 GAE/g. Stability test result found to acceptable and safe for consumption within 1 week only.
Adefegha, S. A., Oboh, G., Molehin, O. R., Saliu, J. A., Athayde, M. L., &Boligon, A. A. (2016). Chromatographic Fingerprint Analysis, Acetylcholinesterase Inhibitory Properties and Antioxidant Activities of RedflowerRagleaf (C rassocephalumCrepidioides) Extract. Journal of food biochemistry, 40(1), 109-119.
Adewale, O. B., Onasanya, A., Anadozie, S. O., Ogbole, C. J., Akintan, I. A., & Abu, M. F. (2015). Protective effect of ethanolic extract of Crassocephalum rubens leaves on carbon tetrachloride-induced liver damage in rats. World J Pharm Med Res, 4, 156-68.
Adjatin, A., Dansi, A., Badoussi, E., Loko, Y. L., Dansi, M., Gbaguidi, F., ...&Sanni, A. (2013). Phytochemical screening and toxicity studies of Crassocephalumrubens (Juss. ex Jacq.) S. Moore and Crassocephalumcrepidioides (Benth.) S. Moore consumed as vegetable in Benin. Journal of Chemical and Pharmaceutical Research, 5(6), 160-167.
Adjatin, A., Dansi, A., Badoussi, E., Sanoussi, A. F., Dansi, M., Azokpota, P., ...&Sanni, A. (2013). Proximate, mineral and vitamin C composition of vegetable Gbolo [Crassocephalumrubens (Juss. ex Jacq.) S. Moore and C. crepidioides (Benth.) S. Moore] in Benin. International Journal of Biological and Chemical Sciences, 7(1), 319-331.
Akakpo, A. D., &Achigan-Dako, E. G. (2019).Nutraceutical Uses of Traditional Leafy Vegetables and Transmission of Local Knowledge from ParenAdjatin, A., Dansi, A., Badoussi, E., Loko, Y. L., Dansi, M.,
Akinpelu, B. A., Godwin, A., Gbadegesin, T., Ajakaye, N., Omotosho, S. E., Azeez, S. O., ...&Oyedapo, O. O. (2019). Comparative Studies on Anti-Inflammatory, Antioxidant and Antimutagenic Activities of Crassocephalumcrepidioides (Bent) Leaf Cold and Hot Water Extracts. Asian Food Science Journal, 1-12.
Ananya Das.,(2017), Evaluation of the local anesthetic effect of Crassocephalumcrepidioides in suitable experimental Animal Models, International Journal of Advanced Research(IIAR) 6(2), 1831-1834.
Arawande, J. O., Komolafe, E. A., &Imokhuede, B. (2013).Nutritional and phytochemical compositions of fireweed (Crassocephalumcrepidioides). Journal of Agricultural Technology, 9(2), 439-449.
Awang-Kanak, F., Bakar, M. F. A., & Mohamed, M. (2019, July).Ethnobotanical note, total phenolic content, total flavonoid content, and antioxidative activities of wild edible vegetable, Crassocephalumcrepidioides from Kota Belud, Sabah. In IOP Conference Series: Earth and Environmental Science (Vol. 269, No. 1, p. 012012). IOP Publishing..
Ayodele, O. O., Onajobi, F. D., &Osoniyi, O. (2019).In vitro anticoagulant effect of Crassocephalumcrepidioides leaf methanol extract and fractions on human blood. Journal of experimental pharmacology, 11, 99.
Ayodele, O. O., Onajobi, F. D., &Osoniyi, O. R. (2020).Modulation of Blood Coagulation and Hematological Parameters by Crassocephalumcrepidioides Leaf Methanol Extract and Fractions in STZ-Induced Diabetes in the Rat. The Scientific World Journal, 2020.Aziz, A., Khan, I. A., Afzal, A., &Munawar, S. H. (2013). Formulation and evaluation of herbal Antitussive syrup of methanolic extract of Lycopuseuropaeus in mice. American journal of Pharmacy& Health Research, 1, 121-128.
Bahar, E., Siddika, M. S., Nath, B., & Yoon, H. (2016).Evaluation of in vitro antioxidant and in vivo antihyperlipidemic activities of methanol extract of aerial Part of Crassocephalumcrepidioides (Asteraceae) Benth S Moore. Tropical Journal of Pharmaceutical Research, 15(3), 481-488Belcher, R. O. (1955).The Typification of Crassocephalum Moench and Gynura Cass. Kew Bulletin, 10(3), 455-465.
Bello Oluwasesan, M., Abiodun, B. O., &Uduma, A. U. Antioxidant and Lipoxygenase Inhibitory Activity of Crassocephalumcrepidioides (Benth.) S. Moore; an Underutilized Vegetable from Nigeria.
Calvin, B. Z., Abiodoun, O. P., Géorcelin, A. G., Laure, N. E., Bertrand, D. A., Blaise, A. A. G., & Sylvie, H. A. (2016). In vitro anthelmintic activity of aqueous extract of Crassocephalumcrepidioides (Benth.) S. Moore on Haemonchuscontortus. Journal of Experimental & Integrative Medicine, 6(1).
Colle, D., Arantes, L. P., Gubert, P., da Luz, S. C. A., Athayde, M. L., Teixeira Rocha, J. B., &Soares, F. A. A. (2012). Antioxidant properties of Taraxacumofficinale leaf extract are involved in the protective effect against hepatoxicity induced by acetaminophen in mice. Journal of medicinal food, 15(6), 549-556.
Dairo, F. A. S., &Adanlawo, I. G. (2007).Nutritional quality of Crassocephalumcrepidioides and Seneciobiafrae. Pak J Nutr, 6(1), 35-39.
Devaraj, E. (2016). Hepatoprotective properties of Dandelion: recent update. Journal of Applied Pharmaceutical Science, 6(04), 202-205.